Lista dos Principais CEPs Nacionais
O Comitê de Ética em Pesquisa (CEP) é um colegiado interdisciplinar e independente, com “munus público”, que deve existir nas instituições que realizam pesquisas envolvendo seres humanos no Brasil, criado para defender os interesses dos sujeitos em sua integridade e dignidade e para contribuir no desenvolvimento da pesquisa dentro dos padrões éticos (Normas e Diretrizes Regulamentadoras da Pesquisa Envolvendo Seres Humanos – Resolução CNS 196/96, II.4). Clique aqui >>
As células só poderão ser requisitadas por pesquisadores exercendo suas atividades em território nacional. O(a) pesquisador(a) deverá enviar para resumo de pesquisa contemplando o uso pretendido para as linhagens, link para CV Lattes atualizado, cópia digitalizada do aceite do projeto em CEP nacional e formulário (disponível no site) preenchido. Clique aqui >>
Generation of 5 hiPSC lines derived from three unrelated idiopathic Parkinson disease patients and two unrelated healthy control individuals
Fabiano de Araújo Tofoli, Hsin Fen Chien, Egberto Reis Barbosa, Lygia V. Pereira
We describe generation of human induced pluripotent stem cell (hiPSC) lines of three unrelated idiopathic late onset Parkinson disease patients and two healthy controls above 60 years of age without neurological diseases nor Ashkenazi ancestry. Human iPSC were derived from peripheral blood-erythroblasts using integration free episomal plasmids carrying four reprogramming factors OCT4, SOX2, c-MYC, KLF4 and BCL-XL. The hiPSC lines were characterized according to established criteria. Clique aqui >>
Generation of three control iPS cell lines for sickle cell disease studies by reprogramming erythroblasts from individuals without hemoglobinopathies
Bruno Diaz Paredes, Gabriele Louise Soares Martins, Carine Machado Azevedo, Gabriela Louise de Almeida Sampaio, Carolina Kymie Vasques Nonaka, Katia Nunes da Silva, Milena Botelho Pereira Soares, Ricardo Ribeiro dos Santos, Bruno Solano de Freitas Souza.
Sickle cell disease (SCD) is one of the most prevalent and severe monogenetic disorders. Previously, we generated iPS cell lines from SCD patients. Here, we generated iPS cell lines from three age-, ethnicity- and gender-matched healthy individuals as control cell lines. Cell reprogramming was performed using erythroblasts expanded from PBMC by a non-integrative method. SCD-iPSC controls expressed pluripotency markers, presented a normal karyotype, were able to differentiate into the three germ layers in embryoid body spontaneous differentiation and confirmed to be integration-free. The cell lines generated here may be used as matched healthy controls for SCD studies. Clique aqui >>
Generation of integration-free iPS cell lines from three sickle cell disease patients from the state of Bahia, Brazil
Gabriele Louise Soares Martins, Bruno Diaz Paredes, Carine Machado Azevedo, Gabriela Louise De Almeida Sampaio, Carolina Kymie Vasques Nonaka, Bruno Raphael Ribeiro Cavalcante, Katia Nunes Da Silvaa, Ciro Silveira E. Pereira, Milena Botelho Pereira Soares, Ricardo Ribeiro Dos Santos, Bruno Solano De Freitas Souza.
Sickle cell disease (SCD) is one of the most prevalent and severe monogenetic disorders, affecting several million people around the world. Clinical manifestations and complications of the disease include sickle cell pain crisis, silent cerebral infarct, stroke, nephropathy and early death. In this study, we generated induced pluripotent stem cell (iPSC) lines from three homozygous SCD patients from the state of Bahia, Brazil, where SCD is highly prevalent. Peripheral blood mononuclear cells were collected and erythroblasts were expanded for cell reprogramming with the use of non-integrative episomal vectors. The generated iPSC lines expressed high levels of pluripotency markers, presented a normal karyotype and were able to differentiate into the three germ layers in embryoid body spontaneous differentiation assays. Moreover, the expression of the episomal vectors was lost in all iPSC lines after 15 passages. These iPSC lines may help increasing the knowledge about SCD pathogenesis and can be a useful tool for drug testing and gene editing studies. Clique aqui >>
Generation of patient-specific induced pluripotent stem cell lines from one patient with Jervell and Lange-Nielsen syndrome, one with type 1 long QT syndrome and two healthy relatives
Kasai-Brunswick T.H., Silva dos Santos D., Ferreira R.P., Araujo D.S., Dias G.M., Coutinho J.L.A., CruzF.E.S.F., Sternick E.B., Gubert F., Oliveira J.C.G., Vaz I.M., Borgonovo T., Brofman P.R.S., Moura-Neto R.S., Silva R., Campos-de-Carvalho A.C., Carvalho A.B..
Four human iPSC cell lines (one Jervell and Lange-Nielsen Syndrome, one Long QT Syndrome-type 1 and two healthy controls) were generated from peripheral blood obtained from donors belonging to the same family. CytoTune™-iPS 2.0 Sendai Reprogramming Kit (containing OCT3/4, KLF4, SOX2 and cMYC as reprogramming factors) was used to generate all cell lines. The four iPSCs have normal karyotype, express pluripotency markers as determined by RT-PCR and flow cytometry and differentiated spontaneously in vitro into cells of the three germ layers, confirming their pluripotent capacity. Clique aqui >>
Generation of iPS cell lines from schizophrenia patients using a non-integrative method
Jaroslaw Sochacki, Sylvie Devalle, Marcelo Reis, Renata de Moraes Maciel, Bruna da Silveira Paulsen, Helena Brentani, Paulo Silva Belmonte-de-Abreu, Stevens Rehen.
Skin biopsies were collected from three male patients (age 45, 44 and 44) with clinically diagnosed schizophrenia. The patients were diagnosed according to DSM-5 criteria by a trained psychiatrist. Dermal fibroblast cell lines were established and expanded for subsequent reprogramming procedures. Induced pluripotent stem (iPS) cells were derived using the integration-free CytoTune®-iPS 2.0 Sendai Reprogramming Kit, containing Sendai virus particles of the four Yamanaka factors Oct3/4, Sox2, Klf4 and c-Myc. Clique aqui >>